Intermediate

Part:BBa_K133015

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF215-mCherry-RGD-Histop

CF215 is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215). In combination with CF213 it forms a TLR5 activating protein.

mCherry is a red fluorescent protein that can be used for localisation studies.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 571
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 128
    Illegal BamHI site found at 639
    Illegal BamHI site found at 1603
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1638


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Categories
Parameters
None